Thanks to the advancement in synthetic biology and DNA manipulation technologies, it is now possible to design new metabolic pathways for producing complex pharmaceuticals in microorganisms with easy growth and handling. Those advancements together with the newest developments in biochemical engineering to design cheaper bioprocesses have a great potential to revolutionise the way pharmaceuticals are produced.
Applications are now accepted for a unique PhD candidate to work in the interphase between synthetic biology and biochemical engineering.
The objective of the project will be to develop and optimise microbial cell factories (genetic engineering of yeast and bacteria) in such a way that they could be utilised in novel cheaper bioreactors (continuous flow) to produce high value natural products with pharmaceutical applications including anti-cancer drugs (for example Taxol) or HIV drugs among others.
This is an interdisciplinary project based in the Institute for Bioengineering (Engineering School of Edinburgh) involving strong collaborations with different laboratories in the Biological School of Edinburgh. The candidate will also become a member of the Centre for Synthetic and Systems Biology (SynthSys), which allows plenty collaboration opportunities with other researchers as well as startups and industry.
The PhD candidate will have the opportunity to use state of the art automated microscale facilities (like the Edinburgh Genome Foundry) to create and screen his library of microbial cell factories.
The candidate will also have the access to many labs and facilities from the School of Biological Sciences for molecular biology experiments as well as proteomics and metabolomics. Full access to the engineering school facilities will also be available to design and build novel bioreactors and bioprocesses.
This project has strong collaborations with third world countries specifically those from Latin America (Mexico) with the aim to transfer the technology so that local communities could produce their own medicines, promoting the creation of jobs using sustainable engineering.
Some specific objectives of the project are stated as follow:
Identification of native pathways and genetic material of interest for the biosynthesis of pharmaceuticals using bioinformatics tools.
Transcriptome-enabled discovery of key enzymes using plant cell cultures combined with novel inducers.
In vivo mutagenesis and protein engineering for pathway optimisation.
Identification of selected genes of interest to improve the performance of cells in continuous flow bioreactors (resistance to sheer stress, biofilm formation, external product transport and resistance to harsher feedstock).
Optimisation of recombinant expression of selected enzymes in non-native hosts.
Development of automated tools for rapid construction and genome integration of complete synthetic metabolic pathways (this will be done in collaboration with the Edinburgh Genome Foundry)
Design of biosensors to apply selective pressure for in vivo optimisation of the pathways.
Design of novel continuous flow bioreactors to host the engineered cells (in collaboration with selected members from the School of Engineering)
Kinetic modelling and characterisation of the pathways using microscale automated tools.
Please note that, if applying for this position, a Research Proposal is not required.
Minimum entry qualification - an Honours degree at 2:1 or above (or International equivalent) in a relevant science or engineering discipline, possibly supported by an MSc Degree.
Further information on English language requirements for EU/Overseas applicants.
Students with background in the selected disciplines are encouraged to apply:
- Chemical/Biochemical Engineering
- Industrial microbiology
- Molecular Biology/ Synthetic biology
Tuition fees and stipend are available for home/EU students. International students can apply, the stipend will be available however the funding will cover the home/EU tuition fee rate only. Applications are also welcomed from self-funded students, or students who are applying to scholarships from the University of Edinburgh or elsewhere.